Extended Title: Flow cytometry analysis of prepared samples (DAPI staining method) to determine prepared larval catfish fish tissue karyotype.
Flow cytometry analysis of prepared samples (DAPI staining method) to determine prepared larval catfish fish tissue karyotype.
Cells are prepared using the CyStain® PI Absolute P reagent kit and modified
protocol of Sysmex®. A pre-calibrated flow cytometer (BD FACSMelody,
Bd Biosciences, San Jose, USA) must be used for determining ploidy.
Larval batches must be separately calibrated with diploid control. About 58
hours required for flow cytometry measurements conducted by a trained
flow cytometer operating expert. Flow cytometry laboratory must be NLA
Bids obtainable from: TumekaNC@daff.gov.za, Cost of Documents: No
Cost, Payment Details: N/A
For technical information please contact: Chris Fouche. Tel: (021)
4307054 Email: ChrisF@daff.gov.za. Hours: 07:00 - 15:30.
For completion of bid documents please contact: Tumeka Ncobo, Tel: 021
402 3382, Email: TumekaNC@daff.gov.za, Hours: 07:30 - 16:00.
Western Cape: Department of Agriculture, Forestry and Fisheries: Aquaculture Research & Development
Tender number: RFQ000408
Closing date and time: 2020-10-30 at 11:00